Primer-Blast results - National Center for Biotechnology Information
Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.
Topical pimecrolimus: a review of its use in the management of
Topical pimecrolimus 1% cream (Elidel) [hereafter referred to as topical pimecrolimus] is a nonsteroidal alternative in the treatment of pediatric atopic dermatitis. In vehicle-controlled, short-term, continuous …
Primer-BLAST Archives - NCBI Insights
To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers. The longest sequence is also …
Basic Local Alignment Search Tool - BLAST
Jul 21, 2025 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and …
Primer-BLAST: a tool to design target-specific primers for ... - PubMed
Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity …
Neuropathic pain - A clinical primer - PubMed
Nov 24, 2024 · Neuropathic pain is used both as a mechanistic descriptor and a classification category of pain caused by a lesion or disease of the somatosensory nervous system and encompasses a …
National Center for Biotechnology Information
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Primer design for RT-PCR - PubMed
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency …
Successful treatment of chronic persistent vesicular hand dermatitis ...
Hand dermatitis is a common chronic skin condition that has many clinical forms including contact, hyperkeratotic, frictional, nummular, atopic, pompholyx and chronic vesicular hand dermatitis. …
Spotlight on topical pimecrolimus in pediatric atopic dermatitis
Topical pimecrolimus 1% cream (Elidel) [hereafter referred to as topical pimecrolimus] is a nonsteroidal alternative in the treatment of pediatric atopic dermatitis. In vehicle-controlled, short-term, continuous …